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聯(lián)系我時,請告知來自 儀表網(wǎng)小鼠25羥基*3(25(OH)D3/25HVD3)elisa免疫檢測試
小鼠β內(nèi)啡肽受體(β-EPR)ELISa免疫檢測試劑盒運輸
保存及運輸溫度:2-8℃低溫避光保存公司批次ELISA試劑盒供應(yīng),常見ELISA試劑盒享受折扣優(yōu)惠。ELISA試劑盒棋盤試驗的操作方法:(1)將抗原按一定的梯度倍比稀釋后,按照ELISA從上到下(或者從左到右)的順序包被。(2)將抗體按一定的梯度倍比稀釋后按照ELISA從左到右或者從上到下加入。(3)二抗的稀釋倍數(shù)是一定的,然后顯色,讀值。
產(chǎn)品名稱:小鼠β內(nèi)啡肽受體(β-EPR)ELISa免疫檢測試劑盒運輸
英文名稱:MouseBeta-Endorphinreceptor,β-EPRELISA Kit
性狀:盒裝液體。
保存溫度: 2~8℃。
檢測范圍:見說明書靈敏度(以隨貨英文說明書為主)。
說明書:說明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取。
樣品收集、處理及保存方法
1、 血清-----操作過程中避免任何細胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后,1000×g離心10分鐘將血清和紅細胞迅速小心地分離。
2、 血漿-----EDTA、檸檬酸鹽、肝素血漿可用于檢測。1000×g離心30分鐘去除顆粒。
3、 細胞上清液---1000×g離心10分鐘去除顆粒和聚合物。
4、 保存------如果樣品不立即使用,應(yīng)將其分成小部分-70 ℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
注意事項
1. 試劑應(yīng)按標簽說明書儲存,使用前恢復(fù)到室溫。稀稀過后的標準品應(yīng)丟棄,不可保存。
2. 實驗中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。
3. 不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用。保質(zhì)前使用。
4. 使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時,避免使用帶金屬部分的加樣器。
5. 使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6. 洗滌酶標板時應(yīng)充分拍干,不要將吸水紙直接放入酶標反應(yīng)孔中吸水。
7. 底物A應(yīng)揮發(fā),避免長時間打開蓋子。底物B對光敏感,避免長時間暴露于光下。避免用手接觸,有毒。實驗完成后應(yīng)立即讀取OD值。
8. 加入試劑的順序應(yīng)*,以保證所有反應(yīng)板孔溫育的時間一樣。
ELISA Kit for Low Density Lipoprotein Receptor Related Protein 6 (LRP6)規(guī)格:48T/96T
ELISA Kit for Uromodulin (UMOD)規(guī)格:48T/96T
ELISA Kit for Ephrin B2 (EFNB2)規(guī)格:48T/96T
ELISA Kit for Alkaline Phosphatase, Intestinal (ALPI)規(guī)格:48T/96T
ELISA Kit for Fibroblast Growth Factor 11 (FGF11)規(guī)格:48T/96T
ELISA Kit for Fibroblast Growth Factor 12 (FGF12)規(guī)格:48T/96T
ELISA Kit for VGF Nerve Growth Factor Inducible (VGF)規(guī)格:48T/96T
ELISA Kit for Heat Shock Protein Beta 8 (HSPb8)規(guī)格:48T/96T
ELISA Kit for Nexilin (NEXN)規(guī)格:48T/96T
ELISA Kit for Kininogen 1 (KNG1)規(guī)格:48T/96T
ELISA Kit for Transmembrane Protein 27 (TMEM27)規(guī)格:48T/96T
ELISA Kit for Transmembrane Protein 27 (TMEM27)規(guī)格:48T/96T
ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK)規(guī)格:48T/96T
ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)規(guī)格:48T/96T
ELISA Kit for Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1)規(guī)格:48T/96T
ELISA Kit for Fibroblast Growth Factor 13 (FGF13)規(guī)格:48T/96T
ELISA Kit for Leucine Aminopeptidase (LAP)規(guī)格:48T/96T
ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)規(guī)格:48T/96T
ELISA Kit for Glutaminase (GLS)規(guī)格:48T/96T
ELISA Kit for Glutaminase 2 (GLS2)規(guī)格:48T/96T
ELISA Kit for Myo Inositol Oxygenase (MIOX)規(guī)格:48T/96T
ELISA Kit for Lamin A/C (LMNA)規(guī)格:48T/96T
ELISA Kit for Aminolevulinate Delta Synthase 1 (ALAS1)規(guī)格:48T/96T
ELISA Kit for Alpha-1-B-Glycoprotein (a1BG)規(guī)格:48T/96T
ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)規(guī)格:48T/96T
ELISA Kit for Hedgehog Homolog, Indian (IHH)規(guī)格:48T/96T
ELISA Kit for Fibulin 1 (FBLN1)規(guī)格:48T/96T
ELISA Kit for NK6 Homeobox Protein 1 (NKX6-1)規(guī)格:48T/96T
ELISA Kit for Desmoglein 2 (DSG2)規(guī)格:48T/96T
ELISA Kit for Arginine Vasopressin Receptor 1B (AVPR1B)規(guī)格:48T/96T
ELISA Kit for Coiled Coil Domain Containing Protein 3 (CCDC3)規(guī)格:48T/96T
ELISA Kit for Fatty Acid Desaturase 2 (FADS2)規(guī)格:48T/96T
ELISA Kit for Fatty Acid Desaturase 2 (FADS2)規(guī)格:48T/96T
ELISA Kit for Fatty Acid Desaturase 1 (FADS1)規(guī)格:48T/96T
ELISA Kit for Fatty Acid Desaturase 1 (FADS1)規(guī)格:48T/96T
ELISA Kit for Fc Fragment Of IgA Receptor (FcaR)規(guī)格:48T/96T
ELISA Kit for Glutamate Decarboxylase Like Protein 1 (GADL1)規(guī)格:48T/96T
ELISA Kit for Wilms Tumor Protein (WT1)規(guī)格:48T/96T
小鼠β內(nèi)啡肽受體(β-EPR)ELISa免疫檢測試劑盒運輸
ELISA Kit for Fatty Acid Desaturase 3 (FADS3)規(guī)格:48T/96T
ELISA Kit for Glutaminyl Peptide Cyclotransferase (QPCT)規(guī)格:48T/96T
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10A (WNT10A)規(guī)格:48T/96T
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 7B (WNT7B)規(guī)格:48T/96T
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 7A (WNT7A)規(guī)格:48T/96T
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)規(guī)格:48T/96T
ELISA Kit for Integrin Associated Protein (IAP)規(guī)格:48T/96T
ELISA Kit for Nucleoporin 62kDa (NUP62)規(guī)格:48T/96T
ELISA Kit for Nuclear Respiratory Factor 1 (NRF1)規(guī)格:48T/96T
ELISA Kit for Hedgehog Homolog, Desert (DHH)規(guī)格:48T/96T
ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)規(guī)格:48T/96T
ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)規(guī)格:48T/96T
ELISA Kit for Endosulfine Alpha (ENSa)規(guī)格:48T/96T
ELISA Kit for Reelin (RL)規(guī)格:48T/96T
ELISA Kit for Kallikrein 12 (KLK12)規(guī)格:48T/96T
ELISA Kit for Kallikrein 14 (KLK14)規(guī)格:48T/96T
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