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聯(lián)系我時,請告知來自 儀表網(wǎng)小鼠25羥基*3(25(OH)D3/25HVD3)elisa免疫檢測試
小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISa免疫檢測試劑盒操作步驟檢測范圍:
人、綿羊、小鼠、大鼠、豬、兔、山羊、牛、馬、豬、其它動物細胞因子、植物細胞因子、骨代謝、細胞凋亡、激素內(nèi)分泌、活性多肽、肝纖維化、自身抗體、血栓與止血、腫瘤、自身抗體科研Elisa檢測試劑盒。
產(chǎn)品名稱:小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISa免疫檢測試劑盒操作步驟
英文名稱:Mouseamyloidbetapeptide1-42,Aβ1-42ELISA Kit
產(chǎn)品性狀:盒裝液體。
運輸條件:2-8℃低溫運輸,用干冰或者冰袋低溫運輸。
待檢樣本:體液、血清、血漿、細胞培養(yǎng)上清液、尿液、組織勻漿、心房水標本等等。
小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISa免疫檢測試劑盒操作步驟原理:ELISA是以免疫學反應(yīng)為基礎(chǔ),將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗技術(shù)。
ELISA試劑盒檢測類型:
雙抗體夾心法測抗原、雙抗原夾心法測抗體、間接法測抗體、競爭法測抗體、競爭法測抗原、捕獲包被法測抗體、ABS-ELISA法。
類型和操作步驟
ELISA可用于測定抗原,也可用于測定抗體。在這種測定方法中有3種必要的試劑:
①固相的抗原或抗體,
②酶標記的抗原或抗體,
③酶作用的底物。
根據(jù)試劑的來源和標本的性狀以及檢測的具備條件,可設(shè)計出各種不同類型的檢測方法。
(一)雙抗體夾心法、
(二)一步法、
(三)間接法測抗體、
(四)競爭法。
優(yōu)點:
1、*抗體——高效、靈敏、特異
2、規(guī)范包被操作——吸附均勻,吸附性好,空白值低,孔底透明度高
3、*的優(yōu)化方案——重復性高,可靠性強
4、技術(shù)服務(wù)要求:專業(yè),規(guī)范,高效
5、適用于體液、組織勻漿液、細胞培養(yǎng)上清液、尿液等多種類型的樣本適用范圍:此試劑盒僅用于科研實驗,禁用于臨床
技術(shù)原理:
(1). 抗原或抗體的固相化及抗原或抗體的酶標記。
(2). 結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學活性。
(3). 酶標記的抗原或抗體既保留其免疫學活性,又保留酶的活性。
(4). 受檢標本與固相載體表面的抗原或抗體起反應(yīng)。再加入酶標記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上。
(5). 此時固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例。
(6). 加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進行定性或定量分析。測定方法具有很高的敏感度(pg-ng/ml水平),并且重復性好。以免疫學反應(yīng)為基礎(chǔ),將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗技術(shù)。
ELISA Kit for Anthrax Toxin Receptor 2 (ANTXR2)規(guī)格:48T/96T
ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6)規(guī)格:48T/96T
ELISA Kit for Angiopoietin Like Protein 4 (ANGPTL4)規(guī)格:48T/96T
ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3)規(guī)格:48T/96T
ELISA Kit for Neogenin 1 (NEO1)規(guī)格:48T/96T
ELISA Kit for Ferritin (FE)規(guī)格:48T/96T
ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg)規(guī)格:48T/96T
小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISa免疫檢測試劑盒操作步驟ELISA Kit for ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)規(guī)格:48T/96T
ELISA Kit for Catechol-O-Methyltransferase (COMT)規(guī)格:48T/96T
ELISA Kit for Neuritin 1 (NRN1)規(guī)格:48T/96T
ELISA Kit for Plaet Activating Factor (PAF)規(guī)格:48T/96T
ELISA Kit for Pim-1 Oncogene (PIM1)規(guī)格:48T/96T
ELISA Kit for Pim-3 Oncogene (PIM3)規(guī)格:48T/96T
ELISA Kit for Pim-2 Oncogene (PIM2)規(guī)格:48T/96T
ELISA Kit for Carboxylesterase 1 (CES1)規(guī)格:48T/96T
ELISA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7)規(guī)格:48T/96T
ELISA Kit for Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)規(guī)格:48T/96T
ELISA Kit for Collagen Type XII (COL12)規(guī)格:48T/96T
ELISA Kit for Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2)規(guī)格:48T/96T
ELISA Kit for Ki-67 Protein (Ki67P)規(guī)格:48T/96T
ELISA Kit for Dual Specificity Phosphatase 5 (DUSP5)規(guī)格:48T/96T
ELISA Kit for Calbindin (CALB)規(guī)格:48T/96T
ELISA Kit for Biglycan (BGN)規(guī)格:48T/96T
ELISA Kit for Neuroserpin (NSP)規(guī)格:48T/96T
ELISA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1)規(guī)格:48T/96T
ELISA Kit for Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1)規(guī)格:48T/96T
ELISA Kit for Dual Specificity Phosphatase 1 (DUSP1)規(guī)格:48T/96T
ELISA Kit for Reticulocalbin 2 (RCN2)規(guī)格:48T/96T
ELISA Kit for Electron Transferring Flavoprotein Dehydrogenase (ETFDH)規(guī)格:48T/96T
ELISA Kit for Low Density Lipoprotein Receptor Related Protein 1 (LRP1)規(guī)格:48T/96T
ELISA Kit for Cytochrome P450 26A1 (CYP26A1)規(guī)格:48T/96T
ELISA Kit for Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)規(guī)格:48T/96T
ELISA Kit for Cytochrome P450 3A4 (CYP3A4)規(guī)格:48T/96T
ELISA Kit for Aspartate Aminotransferase 2 (AST2)規(guī)格:48T/96T
ELISA Kit for Nucleoredoxin (NXN)規(guī)格:48T/96T
ELISA Kit for Retinoschisin (RS)規(guī)格:48T/96T
ELISA Kit for Cholesterol-25-Hydroxylase (CH25H)規(guī)格:48T/96T
ELISA Kit for Cystathionine Beta Synthase (CbS)規(guī)格:48T/96T
ELISA Kit for Regenerating Islet Derived Protein 3 Alpha (REG3a)規(guī)格:48T/96T
ELISA Kit for MARCKS Related Protein (MARCKSL1)規(guī)格:48T/96T
ELISA Kit for L1-Cell Adhesion Molecule (L1CAM)規(guī)格:48T/96T
ELISA Kit for Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10)規(guī)格:48T/96T
ELISA Kit for Dipeptidyl Peptidase 9 (DPP9)規(guī)格:48T/96T
ELISA Kit for Dipeptidyl Peptidase 8 (DPP8)規(guī)格:48T/96T
ELISA Kit for Vesicular Acetylcholine Transporter (VAChT)規(guī)格:48T/96T
ELISA Kit for Nuclear Factor Kappa B (NFkB)規(guī)格:48T/96T
ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)規(guī)格:48T/96T
ELISA Kit for Elastase 2B (ELA2B)規(guī)格:48T/96T
ELISA Kit for Angiopoietin 1 (ANGPT1)規(guī)格:48T/96T
ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 9 (ADAMTS9)規(guī)格:
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