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小鼠抗小核糖核蛋白/Sm抗體(snRNP/Sm)ELISa免疫檢測試劑盒實(shí)驗(yàn)方法保存及運(yùn)輸溫度:2-8℃低溫避光保存公司批次ELISA試劑盒供應(yīng),常見ELISA試劑盒享受折扣優(yōu)惠。ELISA試劑盒棋盤試驗(yàn)的操作方法:(1)將抗原按一定的梯度倍比稀釋后,按照ELISA從上到下(或者從左到右)的順序包被。(2)將抗體按一定的梯度倍比稀釋后按照ELISA從左到右或者從上到下加入。(3)二抗的稀釋倍數(shù)是一定的,然后顯色,讀值。
產(chǎn)品名稱:小鼠抗小核糖核蛋白/Sm抗體(snRNP/Sm)ELISa免疫檢測試劑盒實(shí)驗(yàn)方法
英文名稱:Mousesmallnuclearribonucleoprotein,snRNP/SmELISA Kit
性狀:盒裝液體。
保存溫度: 2~8℃。
檢測范圍:見說明書靈敏度(以隨貨英文說明書為主)。
說明書:說明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取。
樣品收集、處理及保存方法
1、 血清-----操作過程中避免任何細(xì)胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后,1000×g離心10分鐘將血清和紅細(xì)胞迅速小心地分離。
2、 血漿-----EDTA、檸檬酸鹽、肝素血漿可用于檢測。1000×g離心30分鐘去除顆粒。
3、 細(xì)胞上清液---1000×g離心10分鐘去除顆粒和聚合物。
4、 保存------如果樣品不立即使用,應(yīng)將其分成小部分-70 ℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
注意事項(xiàng)
1. 試劑應(yīng)按標(biāo)簽說明書儲(chǔ)存,使用前恢復(fù)到室溫。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄,不可保存。
2. 實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。
3. 不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用。保質(zhì)前使用。
4. 使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時(shí),避免使用帶金屬部分的加樣器。
5. 使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6. 洗滌酶標(biāo)板時(shí)應(yīng)充分拍干,不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。
7. 底物A應(yīng)揮發(fā),避免長時(shí)間打開蓋子。底物B對光敏感,避免長時(shí)間暴露于光下。避免用手接觸,有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。
8. 加入試劑的順序應(yīng)*,以保證所有反應(yīng)板孔溫育的時(shí)間一樣。
ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)規(guī)格:48T/96T
ELISA Kit for 5'-Nucleotidase, Ecto (NT5E)規(guī)格:48T/96T
ELISA Kit for Presenilin 2 (PS2)規(guī)格:48T/96T
ELISA Kit for Bcl2 Associated X Protein (Bax)規(guī)格:48T/96T
ELISA Kit for Adenylate Cyclase 10, Soluble (ADCY10)規(guī)格:48T/96T
ELISA Kit for Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4)規(guī)格:48T/96T
ELISA Kit for Interleukin 22 (IL22)規(guī)格:48T/96T
ELISA Kit for Peroxisome Proliferator Activated Receptor Delta (PPARd)規(guī)格:48T/96T
ELISA Kit for Histone Deacetylase 1 (HDAC1)規(guī)格:48T/96T
ELISA Kit for Parathyroid Hormone Receptor 1 (PTHR1)規(guī)格:48T/96T
ELISA Kit for Ferritin, Light Polypeptide (FTL)規(guī)格:48T/96T
ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)規(guī)格:48T/96T
ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)規(guī)格:48T/96T
ELISA Kit for Ghrelin/Obestatin Preprohormone (GHRL)規(guī)格:48T/96T
ELISA Kit for 20S-Proteasome (20S-PSM)規(guī)格:48T/96T
ELISA Kit for 20S-Proteasome (20S-PSM)規(guī)格:48T/96T
ELISA Kit for Proteasome Subunit Alpha Type 1 (PSMa1)規(guī)格:48T/96T
ELISA Kit for Myosin Heavy Chain 4, Skeletal Muscle (MYH4)規(guī)格:48T/96T
ELISA Kit for Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)規(guī)格:48T/96T
小鼠抗小核糖核蛋白/Sm抗體(snRNP/Sm)ELISa免疫檢測試劑盒實(shí)驗(yàn)方法ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)規(guī)格:48T/96T
ELISA Kit for 5-Hydroxytryptamine Receptor 2A (HTR2A)規(guī)格:48T/96T
ELISA Kit for Heat Shock Protein 90kDa Alpha B1 (HSP90aB1)規(guī)格:48T/96T
ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)規(guī)格:48T/96T
ELISA Kit for Nuclear Receptor Related Protein 1 (NURR1)規(guī)格:48T/96T
ELISA Kit for Adrenergic Receptor Beta 2 (ADRb2)規(guī)格:48T/96T
ELISA Kit for Adrenergic Receptor Beta 3 (ADRb3)規(guī)格:48T/96T
ELISA Kit for Neurofilament, Heavy Polypeptide (NEFH)規(guī)格:48T/96T
ELISA Kit for Sodium/Glucose Cotransporter 1 (SGLT1)規(guī)格:48T/96T
ELISA Kit for Sodium/Glucose Cotransporter 2 (SGLT2)規(guī)格:48T/96T
ELISA Kit for Testis Anion Transporter 1 (TAT1)規(guī)格:48T/96T
ELISA Kit for Regenerating Islet Derived Protein 1 Beta (REG1b)規(guī)格:48T/96T
ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)規(guī)格:48T/96T
ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)規(guī)格:48T/96T
ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)規(guī)格:48T/96T
ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3)規(guī)格:48T/96T
ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)規(guī)格:48T/96T
ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)規(guī)格:48T/96T
ELISA Kit for Histone Deacetylase 4 (HDAC4)規(guī)格:48T/96T
ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)規(guī)格:48T/96T
ELISA Kit for Translocation Associated Membrane Protein 1 (TRAM1)規(guī)格:48T/96T
ELISA Kit for Secretogranin II (SCG2)規(guī)格:48T/96T
ELISA Kit for Adenosine A1 Receptor (ADORA1)規(guī)格:48T/96T
ELISA Kit for Absent In Melanoma 1 (AIM1)規(guī)格:48T/96T
ELISA Kit for Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)規(guī)格:48T/96T
ELISA Kit for ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)規(guī)格:48T/96T
ELISA Kit for TNF Receptor Associated Factor 3 (TRAF3)規(guī)格:48T/96T
ELISA Kit for TNF Receptor Associated Factor 5 (TRAF5)規(guī)格:48T/96T
ELISA Kit for Toll Like Receptor Adaptor Molecule 1 (TICAM1)規(guī)格:48T/96T
ELISA Kit for TANK Binding Kinase 1 (TBK1)規(guī)格:48T/96T
ELISA Kit for V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF)規(guī)格:48T/96T
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