銆€銆€銆�鍎€琛�缍�(w菐ng) 鐮旂櫦(f膩)蹇▕銆戝柈鍒嗗瓙鐔掑厜鍏辨尟鑳介噺杞�(zhu菐n)绉�(FRET)鎶€琛�(sh霉)鏄爺绌剁磵绫冲昂搴︾敓鐗╁垎瀛愬嫊(d貌ng)鎱�(t脿i)鍜岀浉浜掍綔鐢ㄧ殑鍓嶆部鐢熺墿鐗╃悊宸ュ叿銆傚偝绲�(t菕ng)鐨勯暦(zh菐ng)鏅�(sh铆)闁撳柈鍒嗗瓙FRET娓�(c猫)閲忛€氬父渚濊炒浜庡叏鍏�(n猫i)鍙嶅皠鐔掑厜(TIRF)椤井閺�锛岄€氶亷(gu貌)杩借工鍠€�(g猫)鐔掑厜鍒嗗瓙鐨勫挤(qi谩ng)搴﹁畩鍖栵紝鎻ず鍒嗗瓙灞ら潰鐨勫嫊(d貌ng)鎱�(t脿i)淇℃伅銆傜劧鑰岋紝閫欎竴閬�(gu貌)绋嬬敘(ch菐n)鐢熺殑澶ч噺鏁�(sh霉)鎿�(j霉)灏�(du矛)鏁�(sh霉)鎿�(j霉)鍒嗘瀽鍜岀閬告彁鍑轰簡鎸戞埌(zh脿n)銆傚偝绲�(t菕ng)鐨勪汉宸ユ垨鍗婅嚜鍕�(d貌ng)鍖栨柟娉曚笉鍍呮晥鐜囦綆涓嬶紝鑰屼笖瀹规槗寮曞叆涓昏鍋忓樊锛屽毚(y谩n)閲嶄緷璩翠簬瀵�(sh铆)椹�(y脿n)鑰呯殑缍�(j墨ng)椹�(y脿n)銆傛繁搴﹀(xu茅)缈�(x铆)妯″瀷鐨勬噳(y墨ng)鐢ㄥ墖妤靛ぇ鍦版笡灏戜簡浜虹偤閷�(cu貌)瑾�锛屾彁楂樹簡鏁�(sh霉)鎿�(j霉)铏曠悊鐨勯€熷害鍜屾簴(zh菙n)纰烘€с€傜洝绠″姝�锛岀従(xi脿n)鏈夌殑娣卞害瀛�(xu茅)缈�(x铆)妯″瀷寰€寰€闆ｄ互鏍规摎(j霉)鐢ㄦ埗鐨勭壒瀹氶渶姹傞€�(j矛n)琛屽劒(y艒u)鍖�銆�
 

銆€銆€10鏈�28鏃�锛屾竻鑿ぇ瀛�(xu茅)鐢熷懡绉戝(xu茅)瀛�(xu茅)闄㈠拰鍖椾含鐢熺墿绲�(ji茅)妲�(g貌u)鍓嶆部鐮旂┒涓績闄虫槬渚�(l谩i)瑾查绲勫湪銆婇€氳▕·鐢熺墿瀛�(xu茅)銆�(Communications Biology)闆滃織鐧�(f膩)琛ㄤ簡椤岀偤“鍩轰簬娣卞害瀛�(xu茅)缈�(x铆)鍜屽眬閮ㄧ壒寰佺殑鍠垎瀛愮啋鍏変簨浠惰嚜鍕�(d貌ng)璀�(sh铆)鍒ユ柟娉�”(Deep learning based local feature classification to automatically identify single molecule fluorescence events)鐨勮珫鏂�锛屼粙绱逛簡涓€绋悕鐐篋EBRIS(Deep lEarning Based fRagmentatIon approach for Single-molecule fluorescence event identification)鐨勬柊鏂规硶锛屽皥娉ㄤ簬灏�(du矛)鍠垎瀛愮啋鍏夎粚璺＄殑灞€閮ㄧ壒寰侀€�(j矛n)琛屽垎椤�(l猫i)銆傞€氶亷(gu貌)瑾�(di脿o)鏁寸敤鎴跺畾缇╃殑妯�(bi膩o)婧�(zh菙n)锛孌EBRIS鑳藉鍒╃敤鍚屼竴AI妯″瀷锛岀簿纰虹閬稿拰鍒嗘瀽鍥涚ó涓嶅悓椤�(l猫i)鍨嬬殑鍠垎瀛愮啋鍏変簨浠�銆�
 

銆€銆€DEBRIS鐨勬牳蹇冪悊蹇靛湪浜�锛屾墍鏈夐暦(zh菐ng)鏅�(sh铆)闁撳昂搴︾殑鍠垎瀛愯粚璺￠兘鏄敱鏈夐檺椤�(l猫i)鍨嬬殑鍖呭惈鐗瑰畾灞€閮ㄧ壒寰佺殑鐭墖娈垫(g貌u)寤鸿€屾垚鐨勩€傞(l猫i)浼间簬淇勭緟鏂柟濉婁腑锛屾湁闄愮殑绌嶆湪椤�(l猫i)鍨嬪彲浠ユ嫾鎺ュ嚭澶氱ó澶氭ǎ鐨勫京(f霉)闆滅祼(ji茅)妲�(g貌u)銆侱EBRIS妯″瀷涓寘鍚竷鍊�(g猫)椤�(l猫i)鍒ョ殑闆欓€氶亾鐭墖娈靛挤(qi谩ng)搴�-鏅�(sh铆)闁撹贰绶氾紝浠ユā鎿柈鍒嗗瓙闆欒壊鐔掑厜瀵�(sh铆)椹�(y脿n)涓父瑕�(ji脿n)鐨勫眬閮ㄧ壒寰�(鍦�1a)銆傚湪鎳�(y墨ng)鐢ㄦ檪(sh铆)锛孌EBRIS閲囧彇婊戝嫊(d貌ng)绐楀彛閫愬箑闋�(y霉)娓�(c猫)灞€閮ㄧ壒寰�锛岄€欎竴閬�(gu貌)绋嬪皣寮�(qi谩ng)搴�-鏅�(sh铆)闁撹粚璺¤綁(zhu菐n)鎻涚偤妯″紡-鏅�(sh铆)闁撹粚璺�(鍦�1b)銆傞毃鍚庯紝鏍规摎(j霉)鐢ㄦ埗瀹氱京鐨勬(bi膩o)婧�(zh菙n)灏囧叾閫�(j矛n)涓€姝ュ垎椤�(l猫i)鐐轰笉鍚岀祫鍒�锛屽苟璀�(sh铆)鍒ュ柈鍒嗗瓙浜嬩欢銆傜祼(ji茅)鍚堥爯(y霉)娓�(c猫)鐭墖娈电殑灞€閮ㄧ壒寰�銆佹粦鍕�(d貌ng)绐楀彛鎶€琛�(sh霉)鍜岀敤鎴跺畾缇╃殑妯�(bi膩o)婧�(zh菙n)锛孌EBRIS鑳藉閬╂噳(y墨ng)浠绘剰妯″紡鍜屼换鎰忛暦(zh菐ng)搴︾殑闆欓€氶亾鐔掑厜杌岃贰锛岀偤鍠垎瀛愮啋鍏変簨浠剁殑鑷嫊(d貌ng)璀�(sh铆)鍒ユ彁渚涗簡涓€绋挤(qi谩ng)澶х殑宸ュ叿銆�
 

鍦�1.DEBRIS鐨勮〒(x霉n)绶撮泦妲�(g貌u)寤哄拰鍩烘湰娴佺▼
 

銆€銆€鐐轰簡瑭�(p铆ng)浼癉EBRIS鍦ㄩ洐鑹茬啋鍏夎粚璺′笂鐨勫垎椤�(l猫i)鎬ц兘锛岀爺绌朵汉鍝′娇鐢ㄤ簡浜旂祫鍠垎瀛愰洐鑹睩RET鏁�(sh霉)鎿�(j霉)闆�(鍦�2)銆備互浜哄伐鍒嗛(l猫i)绲�(ji茅)鏋滀綔鐐哄熀婧�(zh菙n)鏅�(sh铆)锛孌EBRIS鐛插緱鐨凢RET鍒嗗竷鑸囦汉宸ュ垎椤�(l猫i)绲�(ji茅)鏋滅浉浼兼€ч仈(d谩)鍒�99%銆傞€欎竴绲�(ji茅)鏋滃嚫椤簡DEBRIS鐨勬簴(zh菙n)纰烘€�锛屽悓鏅�(sh铆)椤ず鍑篋EBRIS鍦ㄦ晥鐜�銆佸瑙€鎬у拰鍙噸寰�(f霉)鎬ф柟闈㈠叿鏈夐’钁楀劒(y艒u)鍕�(sh矛)銆�
 

鍦�2.DEBRIS鍒嗛(l猫i)鎬ц兘瑭�(p铆ng)浼�
 

銆€銆€鐮旂┒浜哄摗鍒╃敤DEBRIS棣栨瀵�(sh铆)鐝�(xi脿n)灏�(du矛)鍕�(d貌ng)鎱�(t脿i)鍑虹従(xi脿n)鐨勭啋鍏変俊铏�(h脿o)鐨勬簴(zh菙n)纰鸿瓨(sh铆)鍒ャ€傚嫊(d貌ng)鎱�(t脿i)鍠垎瀛愮啋鍏変俊铏�(h脿o)鏄寚鍦ㄩ噰闆嗛枊(k膩i)濮嬫檪(sh铆)骞朵笉瀛樺湪鐔掑厜淇¤櫉(h脿o)锛屼絾鐢变簬鎿�(ku貌)鏁ｇ殑妯�(bi膩o)瑷樺垎瀛愯垏琛ㄩ潰鍥哄畾鐨勫垎瀛愮浉浜掍綔鐢ㄥ皫(d菐o)鑷寸啋鍏変俊铏�(h脿o)鍦ㄩ噰闆嗛亷(gu貌)绋嬩腑鍑虹従(xi脿n)(鍦�3)銆備緥濡�锛屽湪鐮旂┒Cas12a灏�(du矛)DNA鐨勫垏鍓查亷(gu貌)绋嬩腑锛屽彲浠ュ嫊(d貌ng)鎱�(t脿i)鎹曟崏鍒癈as12a绲�(ji茅)鍚堛€佸垏鍓插拰DNA鐗囨瑙ｉ洟绛変竴绯诲垪鍕�(d貌ng)鎱�(t脿i)閬�(gu貌)绋�(鍦�3a)銆備絾宸茬櫦(f膩)琛ㄧ殑娣卞害瀛�(xu茅)缈�(x铆)妯″瀷灏氱劇(w煤)娉曟湁鏁堣檿鐞嗘椤�(l猫i)鍕�(d貌ng)鎱�(t脿i)鍠垎瀛愮啋鍏変俊铏�(h脿o)銆傜爺绌朵汉鍝″睍绀轰簡DEBRIS鍙嚜鍕�(d貌ng)璀�(sh铆)鍒ュ嚭Cas12a鐨勭祼(ji茅)鍚堝拰鍒囧壊浜嬩欢锛屽苟鍩轰簬鍒嗛(l猫i)绲�(ji茅)鏋滅敓鎴愪簡FRET姒傜巼闅ㄦ檪(sh铆)闁撹畩鍖栧瘑搴﹀湒锛屽彲琛ㄥ緛鍒囧壊閬�(gu貌)绋嬩腑Cas12a鐨勪竴绯诲垪寰�(f霉)闆滄(g貌u)璞¤畩鍖�銆�
 

鍦�3.DEBRIS鍦ㄦ崟鎹夊嫊(d貌ng)鎱�(t脿i)鐔掑厜淇¤櫉(h脿o)涓殑鎳�(y墨ng)鐢�
 

銆€銆€DEBRIS妯″瀷闆栫劧鏄埄鐢ㄩ洐鑹茶贰绶氶€�(j矛n)琛岃〒(x霉n)绶寸殑锛屼絾涔熷彲浠ユ嫇灞曠敤浜庡柈鑹插柈鍒嗗瓙鐔掑厜杌岃贰銆傜爺绌朵汉鍝″皪(du矛)姝や篃閫�(j矛n)琛屼簡椹�(y脿n)璀�(鍦�4)

 

鍦�4.DEBRIS鍦ㄥ柈鑹插柈鍒嗗瓙鐔掑厜杌岃贰涓婄殑鎳�(y墨ng)鐢�
 

銆€銆€DEBRIS閫氶亷(gu貌)绮炬簴(zh菙n)璀�(sh铆)鍒ュ柈鍒嗗瓙鐔掑厜杌岃贰鐨勫眬閮ㄧ壒寰�锛屽苟鍏佽ū鏍规摎(j霉)瀵�(sh铆)椹�(y脿n)瑷�(sh猫)瑷�(j矛)闈堟椿瑾�(di脿o)鏁村垎椤�(l猫i)妯�(bi膩o)婧�(zh菙n)锛屽(sh铆)鐝�(xi脿n)浜嗗湪涓嶄慨鏀圭缍�(j墨ng)缍�(w菐ng)绲�(lu貌)绲�(ji茅)妲�(g貌u)鐨勫墠鎻愪笅锛屽皪(du矛)闆欒壊/鍠壊瀵�(sh铆)椹�(y脿n)姊濅欢涓嬬殑绌�(w臎n)瀹氬拰鍕�(d貌ng)鎱�(t脿i)鍠垎瀛愮啋鍏変俊铏�(h脿o)閫�(j矛n)琛屾簴(zh菙n)纰鸿瓨(sh铆)鍒�銆傞€欑ó鍓�(chu脿ng)鏂扮殑鏂规硶鐐哄柈鍒嗗瓙鐔掑厜鎶€琛�(sh霉)鐨勬噳(y墨ng)鐢ㄦ彁渚涗簡涓€鍊�(g猫)楂樻晥鐨勬暩(sh霉)鎿�(j霉)鍒嗘瀽宸ュ叿锛屾サ澶у湴?c谩i)U(ku貌)灞曚簡鍏跺湪鐢熺墿鐗╃悊鐮旂┒涓殑娼涘姏銆�
 

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