簡介
OX1LP溶解氧測量儀使用極譜溶解氧電極，可用于測量水相中的光合作用和/或呼吸作用，適用于藻類、植物、動物和細(xì)胞器（葉綠體和線粒體）的懸浮液。還可用于監(jiān)測在水相中產(chǎn)生或消耗氧氣的化學(xué)和生化反應(yīng)。溶解氧包配有儀器、附件、軟件和接口。
可提供從100ul（流通）到50ml不同體積的DO電極。水套反應(yīng)杯內(nèi)的氧電極可用于測量生物樣品或酶反應(yīng)的O2消耗（或產(chǎn)生）。
采用簡單的兩點校準(zhǔn)， 可在相同的溫度下進行校準(zhǔn)和實驗，或者可以在軟件中對不同溫度下的測量進行簡單的溫度校正。隨機附校準(zhǔn)所需的所有附件。
測量對象 v 藻類光合作用與呼吸作用 v 細(xì)菌呼吸 v 微生物呼吸 v 小型水生動物的呼吸 v 水生昆蟲和幼蟲呼吸 v 線粒體呼吸 v 葉綠體O2的產(chǎn)生 v 細(xì)胞懸浮呼吸 v 植物細(xì)胞懸浮光合作用 | 應(yīng)用領(lǐng)域 v 水生植物光合氧代謝的測定 v 抑制劑對線粒體電子傳遞的影響 v 光依賴性葉綠體電子輸運分析 v 溫度對水生動物攝氧量的影響 v 酶動力學(xué)耗氧研究 |
基本組件 溶解氧反應(yīng)杯電極（1,2.5,4,6,30,50ml） 反應(yīng)杯電極放大器 磁力攪拌器 攪拌棒和O形圈 KCl填充液 膜材料和濾紙 水套用10英尺Excelon管 LabQuest迷你數(shù)據(jù)接口 Logger Pro軟件 定制安裝軟件 操作手冊 可選組件： 向反應(yīng)杯中添加代謝物的注射器； 10微升，50微升，100微升 樣品溫度測量用熱敏電阻 校準(zhǔn)LED光源 Q-Box堅固外殼，用于儲存和運輸所有部件 |
DO電極，帶磁力攪拌器和LED光源 |
不同體積的溶解氧反應(yīng)杯電極 OX1LP-1 (1ml 有機玻璃) OX1LP-2.5 (2.5ml 玻璃 或有機玻璃) OX1LP-4 (4ml 有機玻璃) OX1LP-6 (6ml 玻璃) OX1LP-30 (30ml 有機玻璃) OX1LP-50 (50ml有機玻璃或玻璃) |
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軟件界面及數(shù)據(jù)
客戶評價
“I have tested my new Qubit system with isolated oyster mitochondria, and was extremely pleased with the results. I can’t believe I was suffering for the whole year with my old chamber. I have tried three different systems based on Clarke-type electrodes including this one, and this is by far the most convenient for use with small sample volumes and gives a very clean, repeatable trace with the best signal to noise ratio I ever had with micro-chambers.”
From Dr. Inna Sokolova, Assistant Professor, Biology Department, University of North Carolina at Charlotte
“我用分離的牡蠣線粒體測試了Qubit系統(tǒng)，對結(jié)果非常滿意。我真不敢相信忍受了整整一年舊培養(yǎng)室。我嘗試了三種不同類型的克拉克電極，包括這一種，這是迄今為止測量小樣本的系統(tǒng)，提供了一種非常干凈、可重復(fù)的軌跡，且在微型培養(yǎng)室中信噪比。”
——北卡羅來納大學(xué)生物系助理教授 Dr. Inna Sokolova
發(fā)表文獻(xiàn)
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· Sussarellu, Rossana, et al. Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569 (2013).
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· Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
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產(chǎn)地：加拿大
