hela 細胞

細胞名稱： hela 細胞 （STR鑒定正確）

細胞別稱：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri

貨號：TCH-C193

規(guī)格： 1X10^6

細胞簡稱：HeLa

種屬來源：人

組織來源：宮頸

疾病特征：宮頸癌

細胞形態(tài)：上皮細胞樣

生長特性：貼壁生長

培養(yǎng)體系：MEM+10%FBS+1%P/S

配套培養(yǎng)基貨號：TCH-G193

傳代比例：1:2-1:6，每2-3天換液一次

hela 細胞傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細菌、真菌、支原體檢測均為陰性

        hela 細胞是一個來自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細胞系，它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。已知該細胞系含有人乳頭狀瘤病毒HPV18序列，需在2級生物安全防護臺操作。該細胞角蛋白陽性，p53表達量較低，但表達正常水平的pRB（視網(wǎng)膜母細胞瘤抑制因子）。

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