背景介紹:This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.
別名:5)-tetrakisphosphate 3-phosphatase; 5)P(4) 3-phosphatase; DKFZp564L2016; HIPER1; Inositol (1; inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase; Ins(1; ins(1,3,4,5)P(4) 3-phosphatase; MINP1_HUMAN; Minpp1; MINPP2; MIPP; Multiple inositol polyphosphate histidine phosphatase, 1; Multiple inositol polyphosphate phosphatase 1; multiple inositol polyphosphate phosphatase 2.
多聚磷酸肌醇磷酸酶1抗體,Anti-MINPP1抗體簡(jiǎn)單介紹:
產(chǎn)品編號(hào):BYK-18942R
英文名稱:Anti-MINPP1
中文名稱:多聚磷酸肌醇磷酸酶1抗體
產(chǎn)品規(guī)格:50ul;100ul;200ul;1ml,*
抗體來(lái)源 Rabbit or Mouse
克隆類型 Polyclonal or Monoclonal
交叉反應(yīng)、產(chǎn)品應(yīng)用 (具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索取)
性 狀 Lyophilized or Liquid
濃 度 1mg/ml
多聚磷酸肌醇磷酸酶1抗體,Anti-MINPP1抗體產(chǎn)品信息:
博研生物小課堂:
常用激光器對(duì)應(yīng)熒光素的選擇:
藍(lán)色激光器熒光素(488nm):Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7
紅色激光器熒光素(633nm):APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647
黃色激光器熒光素(561nm):PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594
實(shí)驗(yàn)流程:
1、獲取*碎或切碎組織為2~4mm碎塊,用1XPBS沖洗一遍,盡量多的去除組織內(nèi)xue塊。
2、將剪碎的組織放在載玻片磨砂邊,滴加兩滴1XPBS, 另取一個(gè)磨砂邊的載玻片進(jìn)行研磨細(xì)胞至粘稠無(wú)顆粒物,過(guò)濾除去細(xì)胞碎片。
3、加PBS 5ml,200g離心5min,棄去上清。重復(fù)洗滌一次,計(jì)數(shù)和活力分析。
4、300g 離心5min,棄去上清,應(yīng)用適量的流式孵育緩沖液重懸細(xì)胞,調(diào)整細(xì)胞濃度為1×107/ml。
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