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SYBR Green 1 熒光染料號:163795-75-3
英文名稱:SYBR Green 1;SYBR Green I nucleic acid gel stain;SDNA-Nucleic acids stain dye
其他名稱:SYBRGREENⅠ核苷酸膠體染料;SDNA核苷酸染液;Green-DNA Dye 核苷酸膠體染料
號:163795-75-3
級別:BR
描述:原液。每微升染料可配制10毫升Agarose gel。操作簡單,無須脫色或沖洗。至少可檢出20 pg DNA,高于EB染色法25~100倍。與dsDNA 結合熒光信號會增強800~1000倍,0.1 ml Green-DNA Dye is sufficient for 20-25 minigels。
性狀(以下信息僅供參考):橙色液體。花青類染料,致突變性明顯低于EB,使用安全。與核酸結合呈現(xiàn)綠色熒光(Z大吸收為470nm),檢測核酸的靈敏度比EB高,也可用紫外燈進行觀測。用常規(guī)乙醇沉淀方法處理核酸時,該染料可全部從雙鏈核酸上去掉。
用途:本品僅供科研,不得用于其它用途。(以下用途僅供參考)SYBR Green 1是高靈敏的DNA熒光染料,適用于各種電泳分析。
保存:-20℃SYBR Green 1 熒光染料號:163795-75-3儲存條件:
避光、干燥陰涼處封閉貯存,嚴禁與有毒、有害物品混放、混運。本品為非危險 產(chǎn)品可按一般化學品運輸,輕搬動輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運輸條件比較苛刻。
運輸:汽車運輸、EMS郵政快遞,申通快遞等, 款到上海3天內(nèi)發(fā)貨;
售后:如您對我們的產(chǎn)品服務及技術指標有特殊要求,請及時通知我方。
存儲:應貯存在干燥清潔避光的環(huán)境中,嚴禁與有毒物質混放,以免污染(保質期為兩年)。
SYBR Green 1 熒光染料號:163795-75-3主要優(yōu)級純、分級純和化學純3種:
(1)優(yōu)級純(GR:Guaranteed reagent),又稱一級品或保證試劑,99.8%,這種試劑純度Z高,雜質含量Z低,適合于重要精密的分析工作和科學研究工作,使用綠色瓶簽。
(2)分析純(AR),又稱二級試劑,純度很高,99.7%,略次于優(yōu)級純,適合于重要分析及一般研究工作,使用紅色瓶簽。
(3)化學純(CP),又稱三級試劑,≥ 99.5%,純度與分析純相差較大,適用于工礦、學校一般分析工作。使用藍色(深藍色)標簽。
(4)實驗試劑(LR:Laboratory reagent),又稱四級試劑。
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DLG4/PSD-95 and GRIN1/NMDAR1.
Tissue Specificity : Expressed in the blood vessel walls (at protein level). Detected in brain, and at lower levels in pancreas islet beta cells.
Similarity : Belongs to the type-B carboxylesterase/lipase family.
英文名稱 Anti-Nucleoporin p62
中文名稱 核孔糖蛋白P62抗體
別 名 NUP62_HUMAN; 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; Nuclear pore glycoprotein p62; nucleoporin 62kDa; nucleoporin p62; nucleoporin p62KD; NUP62; NUP62 protein; Nucleoporin Nup62; p62.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
產(chǎn)品類型 一抗
研究領域 細胞生物 免疫學 染色質和核信號 神經(jīng)生物學 信號轉導 細胞凋亡 生長因子和激素 細胞類型標志物 表觀遺傳學
蛋白分子量 predicted molecular weight: 53kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Nucleoporin p62
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq].
Function : Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.
Subunit : Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUPL1 (By similarity). Interacts with C11orf73/Hikeshi.
Subcellular Location : Nucleus, nuclear pore complex. Cytoplasm, cytoskeleton, spindle pole. Note=Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.
Post-translational modifications : O-glycosylated. Contains about 10 N-acetylglucosamine side chain sites predicted for the entire protein, amongst which only one in the C-terminal.
DISEASE : Infantile striatonigral degeneration (SNDI) [MIM:271930]: Neurological disorder characterized by symmetrical degeneration of the caudate nucleus, putamen, and occasionally the globus pallidus, with little involvement of the rest of the brain. The clinical features include developmental regression, choreoathetosis, dystonia, spasticity, dysphagia, failure to thrive, nystagmus, optic atrophy, and mental retardation. Note=The disease is caused by mutations affecting the gene represented in this entry.
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