英文名稱Rabbit Anti-human IgM/Gold

中文名稱：膠體金標(biāo)記的兔抗人IgM

規(guī)格：0.5ml

說 明 書 0.5ml（10nm  15nm  35nm）

抗體來源Rabbit

克隆類型Polyclonal

交叉反應(yīng) hu

產(chǎn)品應(yīng)用IEM=1:20-200 IGS=1:20-200 GICA=1:20-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量900kDa

性    狀Lyophilized or Liquid

濃    度0.4mg/1ml

免 疫 原Full length plasma protein

亞    型IgG

純化方法affinity purified by Protein A

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C.

產(chǎn)品介紹background:

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

公司提供膠體金標(biāo)記的兔抗人IgM使用說明書質(zhì)量*，產(chǎn)品齊全，保證實(shí)驗(yàn)的有效性，咨詢。 免費(fèi)送貨上門。

膠體金標(biāo)記的兔抗人IgM使用說明書抗體的鑒定
篩選雜交瘤細(xì)胞通過選擇性培養(yǎng)而獲得雜交細(xì)胞系中，僅少數(shù)能分泌針對免疫原的特異性抗體。一般在雜交瘤細(xì)胞布滿孔底1／10面積時，即可開始檢測特異性抗體，篩選出所需要的雜交瘤細(xì)胞系?！?/span>
檢測抗體的方法應(yīng)根據(jù)抗原的性質(zhì)、抗體的類型不同，選擇不同的篩選方法，一般以快速、簡便、特異、敏感的方法為原則。
常用的方法有:
　　1.　ELISA用于可溶性抗原(蛋白質(zhì))、細(xì)胞和病毒等McAb的檢測。
　　2.　RIA用于可溶性抗原、細(xì)胞McAb的檢測。
　　3.　FACS(熒光激活細(xì)胞分類儀)用于檢查細(xì)胞表面抗原的McAb檢測。
　　4.　IFA用于細(xì)胞和病毒McAb的檢測。
可靠的篩選方法必須在融合前建立，避免由于方法不當(dāng)貽誤整個篩選時機(jī)。
應(yīng)用范圍：
   1.免疫組織及免疫病理中抗原、抗體檢查，如：各腫瘤抗原檢查，細(xì)胞凋亡檢測。
   2.用于細(xì)胞因子等檢查的熒光流式細(xì)胞儀測定。
   3.用于臨床自身免疫病的多種自身抗體熒光檢查，如：抗核抗體，抗心肌酶抗體。
   4.白細(xì)胞分型抗原的免疫熒光染色。
   5.用于細(xì)菌、病毒、梅毒螺旋體、鉤端螺旋體等微生物的抗原及抗體檢測。
主要性能：
0.5ml或根據(jù)試驗(yàn)需求的各種包裝，并提供濃縮液或凍干粉，含有0.01%NaN3防腐劑。 β-arrestin 1蛋白IgG含量在2mg/ml。FITC為Sigma異構(gòu)體I，約在70-80ug/ml，495nm為zui大吸收峰，激發(fā)波長 525 nm 。
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