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Fluorescence RNA In Situ Hybridization (RNA FISH)—Overview
Fluorescence based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescence in situ hybridization of RNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. QuantiGene ViewRNA is a novel non-radioactive RNA in situ hybridization solution that offers single-molecule RNA sensitivity for one to four RNA targets. Affymetrix Research-Use-Only (RUO) products for RNA FISH analysis allow investigators doing biomarker discovery or clinical research to validate and study RNA biomarkers in suspension or adherent cells. |
QuantiGene ViewRNA ISH Cell Assay—Unique Benefits
- Sensitive in situ hybridization assay – detect one mRNA molecule/cell
- Multiplex in situ hybridization – study one to four mRNA targets per cell
- Flexible probe set design – to target any mRNA sequence
- Fast results – one day assay
- Fluorescence signal – use of fluorescence microscope (no radioactivity involved)
- Robust, reproducible results in adherent or suspension cells