上海博研生物工程研究中心竭誠為您提供的產(chǎn)品和服務(wù)，期待您的來電,Elisa試劑盒，Elisa試劑盒說明書，Elisa試劑盒價(jià)格
產(chǎn)品名稱：雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體（sEPCR）ELISA試劑盒注意事項(xiàng)
英文名稱：Chicken soluble endothelial protein C receptor,sEPCR ElisaKit.
產(chǎn)品編號(hào)：BYS12694B
產(chǎn)品性狀：液體
產(chǎn)品用途：科研實(shí)驗(yàn)
產(chǎn)品規(guī)格：96T/48T
ELISA實(shí)驗(yàn)原理: 用純化的抗體包被微孔板，制成固相載體，往包被抗體的微孔中依次加入標(biāo)本或者標(biāo)準(zhǔn)品、*化的抗體、HRP-標(biāo)記的親和素，經(jīng)過*洗滌后用底物顯色。底物在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色， 并在酸的作用下轉(zhuǎn)化成zui終的黃色。 顏色的深淺和樣品中的目標(biāo)蛋白呈正相關(guān)。用酶標(biāo)儀在（450nm）波長(zhǎng)下測(cè)定測(cè)定O.D.值（吸光度），計(jì)算樣品濃度。.
雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體（sEPCR）ELISA試劑盒注意事項(xiàng) 相關(guān)產(chǎn)品：
人β2微球蛋白（BMG/β2-MG）試劑盒說明書，96T/48T*
人膜聯(lián)蛋白Ⅴ（ANX-Ⅴ）試劑盒說明書，96T/48T*
人落葉型天皰瘡抗體（PF）試劑盒說明書，96T/48T*
人妊娠皰疹抗體（HG）試劑盒說明書，96T/48T*
人β2糖蛋白1抗體IgA（β2-GP1 Ab IgA）試劑盒說明書，96T/48T*
人β2糖蛋白1抗體IgM（β2-GP1 Ab IgM）試劑盒說明書，96T/48T*
人β2糖蛋白1抗體IgG（β2-GP1 Ab IgG）試劑盒說明書，96T/48T*
人抗心磷脂抗體IgA（ACA-IgA）試劑盒說明書，96T/48T*
人抗心磷脂抗體IgM（ACA-IgM）試劑盒說明書，96T/48T*
人抗心磷脂抗體IgG（ACA-IgG）試劑盒說明書，96T/48T*
兔子*（Cortisol）試劑盒說明書，96T/48T*
兔子腦鈉素/腦鈉尿肽（BNP）試劑盒說明書，96T/48T*
兔子內(nèi)皮抑素（ES）試劑盒說明書，96T/48T*
兔子內(nèi)皮素1（ET-1）試劑盒說明書，96T/48T*
兔子免疫球蛋白M（IgM）試劑盒說明書，96T/48T*
兔子免疫球蛋白G（IgG）試劑盒說明書，96T/48T*
兔子免疫球蛋白E（IgE）試劑盒說明書，96T/48T*
兔子免疫球蛋白A（IgA）試劑盒說明書，96T/48T*
兔子可溶性血管細(xì)胞粘附分子1（sVCAM-1）試劑盒說明書，96T/48T*
兔子可溶性細(xì)胞間粘附分子1（sICAM-1）試劑盒說明書，96T/48T*
The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve
