上海博研生物工程研究中心竭誠為您提供的產(chǎn)品和服務，期待您的來電,Elisa試劑盒，Elisa試劑盒說明書，Elisa試劑盒價格

產(chǎn)品名稱：雞可溶性細胞間粘附分子1（sICAM-1）ELISA試劑盒值得擁有    
英文名稱：Chicken Soluble InteFAellular Adhesion Molecule-1,sICAM-1  ElisaKit.
產(chǎn)品編號：BYS12651B
產(chǎn)品性狀：液體
產(chǎn)品用途：科研實驗
產(chǎn)品規(guī)格：96T/48T

ELISA實驗原理: 用純化的抗體包被微孔板，制成固相載體，往包被抗體的微孔中依次加入標本或者標準品、*化的抗體、HRP-標記的親和素，經(jīng)過*洗滌后用底物顯色。底物在過氧化物酶的催化下轉(zhuǎn)化成藍色， 并在酸的作用下轉(zhuǎn)化成zui終的黃色。 顏色的深淺和樣品中的目標蛋白呈正相關。用酶標儀在（450nm）波長下測定測定O.D.值（吸光度），計算樣品濃度。.

雞可溶性細胞間粘附分子1（sICAM-1）ELISA試劑盒值得擁有  相關產(chǎn)品：

人抗IVIgG抗體試劑盒說明書，96T/48T*
人抗心肌抗體（AMA）試劑盒說明書，96T/48T*
人碳酸酐酶（CA）試劑盒說明書，96T/48T*
人β血小板球蛋白/β血栓環(huán)蛋白（β-TG）試劑盒說明書，96T/48T*
人抗*（AT）試劑盒說明書，96T/48T*
人抗核糖體P蛋白抗體（ARPA/Rib-P）試劑盒說明書，96T/48T*
人組蛋白H2b（histon-H2b）試劑盒說明書，96T/48T*
人甲狀腺球蛋白（TG）試劑盒說明書，96T/48T*
人抗線粒體抗體（AMA）試劑盒說明書，96T/48T*
人可提取核抗原（ENA）試劑盒說明書，96T/48T*
兔子S100蛋白（S-100）試劑盒說明書，96T/48T*
兔主要組織相容性復合體Ⅱ類（MHCⅡ/RLAⅡ）試劑盒說明書，96T/48T*
兔子*受體（TR）試劑盒說明書，96T/48T*
兔子可溶性P選擇素（sP-selectin）試劑盒說明書，96T/48T*
兔子Ⅰ型前膠原羧基端肽（PⅠCP）試劑盒說明書，96T/48T*
兔子Ⅰ型膠原（Col Ⅰ）試劑盒說明書，96T/48T*
兔子Ⅱ型膠原（Col Ⅱ）試劑盒說明書，96T/48T*
兔子Ⅲ型前膠原肽（PⅢNP）試劑盒說明書，96T/48T*
兔子Ⅲ型前膠原氨基端肽（PⅢNT）試劑盒說明書，96T/48T*
兔子*Ⅱ（FⅡ）試劑盒說明書，96T/48T*

The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve