Reagent :Polyplus PEI DNA轉染試劑
Molecule delivered: DNA
Applications : Plasmid transfection
High Throughput Screening (HTS)
Cell types: Adherent and suspension cells
Number of transfections: 1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.
Storage: Store jetPEI® at 5 °C ± 3°C.
Provided with:101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation.
Protocol:
In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.
The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).
The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.
Fig.2 Effect of complex formation incubation time on transfection efficiency with jetPEI®. HEK-293 cells were transfected in 96-well plates with pCMVLuc and jetPEI® following the reverse transfection protocol. Luciferase activity was measured after 24 h.
