進(jìn)行快速動(dòng)力學(xué)瞬間實(shí)時(shí)記錄,或離線分析,為廣大的快速動(dòng)力學(xué)研究學(xué)者提供了強(qiáng)有力的武器??蛇M(jìn)行下列應(yīng)用:
* 蛋白質(zhì)折疊動(dòng)力學(xué)(Protein Folding)
* 酶動(dòng)力學(xué)(Enzyme Kinetice)
* 底物結(jié)合(Substrate Binding)
* 構(gòu)象轉(zhuǎn)變(Conformational Changes)
* 胞內(nèi)底物運(yùn)輸(Substrate Transport in Vesicles)
* 第二信使研究(Second Messenger Studies)
* 快速化學(xué)反應(yīng)動(dòng)力學(xué)(Rapid Chemical Reaction Kinetics)
儀器配置:
(a). Computer-controlled servo motordrive unit. ---------電腦控制的伺服馬達(dá)驅(qū)動(dòng)系統(tǒng)
(b). Syringe/valve/cell assembly withthree drive syringes and choice of 0.2, 0.5 or 2.5 cm path length observationcells; each cell requires less than 20 μl per reactant per shot and has a dead time lessthan 1 msec. -----------3個(gè)驅(qū)動(dòng)針筒、閥、樣品池
(c). Electronic control unit with servomotor power supply and amplification circuits for two channels allowingsimultaneous measurement of fluorescence and absorbance. --------電子控制器,同時(shí)檢測(cè)熒光和吸收雙通道
(d). Arc lamp source consisting of lowripple power supply, 150 watt mercury-xenon lamp, with parabolic mirror housing,monochromator and optical fiber. ----------150瓦汞燈
(e). Two detectors, one UV-enhancedphotodiode and one UV-enhanced photomultiplier. ------------兩個(gè)紫外檢測(cè)器
(f). Dell Precision T3500 MiniTowerwith Intel 2.53 GHz quad core processor---------------電腦
(g). Software for data collection andanalysis, running under Windows XP.--------軟件
光譜儀單色器及光纖已經(jīng)包含,熒光部分(熒光滴定及特異性研究),其它快速動(dòng)力學(xué)瞬間實(shí)時(shí)記錄功能是一致的。
優(yōu)點(diǎn)
1、伺服馬達(dá)驅(qū)動(dòng)系統(tǒng),其它品牌使用氣動(dòng)或步進(jìn)馬達(dá),伺服馬達(dá)驅(qū)動(dòng)在高轉(zhuǎn)矩,閉環(huán)伺服控制方面優(yōu)于其它馬達(dá)系統(tǒng).
*,針對(duì)高轉(zhuǎn)矩, 對(duì)于“停止/開始”瞬間精準(zhǔn)控制,保持多次試驗(yàn),長(zhǎng)時(shí)間試驗(yàn)的高重復(fù)性( from experiment to experiment and from year to year ). 氣動(dòng)系統(tǒng)必須在恒壓強(qiáng)下工作,而不能在恒速度下工作,導(dǎo)致不能長(zhǎng)時(shí)間工作的重復(fù)性; 步進(jìn)馬達(dá)有很強(qiáng)的反沖效應(yīng),導(dǎo)致驅(qū)動(dòng)時(shí)間過長(zhǎng)而不能工作。
第二, 伺服馬達(dá)驅(qū)動(dòng)可以閉環(huán)控制位移,精準(zhǔn)地反饋并修正速度和位置參數(shù),具有*的高重復(fù)性!這是氣動(dòng)或步進(jìn)馬達(dá)所不具備的,它們沒有反饋修正功能的能力。Basically the servo motors combination of instantaneous stops andstarts as well as closed loop feedback ensure unparallel repeatability.
2、三支注射針筒系統(tǒng),更好的順序混合 ( sequential mixing)
氣動(dòng)系統(tǒng)不能很好地順序混合,因?yàn)椴皇峭活愋偷膱?zhí)行觸發(fā)器
Pneumatic systems are quite poor at sequential mixing, since they are an all ornon type of actuator.
3、三支針筒在“開始/停止”操作一致性,保持高重復(fù)性!
其它品牌每一個(gè)針筒采用單獨(dú)的步進(jìn)馬達(dá),同步性較差,步調(diào)不一致,導(dǎo)致數(shù)據(jù)延時(shí),重復(fù)性不好。
4、小體積量20微升,Minimum volume of 20 ul per reactant, per shot.
5、一致性的死時(shí)間.
氣動(dòng)系統(tǒng)必須在恒壓強(qiáng)下工作,而不能在恒速度下工作,導(dǎo)致死時(shí)間變化(因?yàn)橥七M(jìn)器在不斷老化),需要經(jīng)常校準(zhǔn)。
6、兩年保修.
7、高靈敏性的PMT(光電倍增管)檢測(cè)器。
The observation cell, the filter and PMT geometry have been optimized formaximum signal.
8、超級(jí)安靜的電子控制器提高信噪比,使得低亮度的熒光得到檢測(cè)
Ultra quiet electronics for the light source for improved signal to noise forlow light level fluorescence.
9、透明的注射針筒,氣泡可以看見并移除,其它品牌無此功能,只有拿到不好的數(shù)據(jù)時(shí)才知道!.