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澶ф渻(hu矛)鐝�(xi脿n)鍫�(ch菐ng)

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鐩存搳鐢熺墿鍒惰棩鍥涘ぇ鐥涢粸(di菐n)

鐪嬭辰榛橀鑹茶瓬璩�(zh矛)璀滅敓鐗╁埗钘ヨВ姹烘柟妗�

涓€銆�

琛ㄥ緛涓€绱�(j铆)绲�(ji茅)妲�(g貌u)

鐥涢粸(di菐n)1.1

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鏂规

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鐥涢粸(di菐n)1.2

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鏂规

鑲藉湒鐨勬恫璩�(zh矛)瀵�(sh铆)椹�(y脿n)娴佺▼涓昏鍖呮嫭锛氳泲鐧界殑璁婃€�銆佽泲鐧介叾閰惰В锛堥€氬父浣跨敤鑳拌泲鐧介叾 Trypsin锛�銆佹恫璩�(zh矛)鑱�(li谩n)鐢ㄦ暩(sh霉)鎿�(j霉)閲囬泦銆佹暩(sh霉)鎿�(j霉)铏曠悊骞惧€�(g猫)姝ラ銆係MART Digest瑭﹀姂鐩掑惈妯ｅ搧闋�(y霉)铏曠悊瑭﹀姂鍖呮嫭璁婃€�锛岄倓鍘燂紝鐑峰熀鍖栬│鍔�锛屽揩閫熼叾瑙�锛岀祼(ji茅)鏋滅┅(w臎n)瀹�銆侀噸寰�(f霉)鎬уソ銆侽rbitrap楂橀潏鏁忓害鍜岄珮璩�(zh矛)閲忕簿搴︾殑鐗归粸(di菐n)——鍗€(q奴)鍒嗗叡娲楄劔鍜岃唱(zh矛)閲忕浉杩戠殑绲勫垎锛屽悓鏅�(sh铆)灏�(du矛)搴忓垪璁婄暟楂斻€佸悗淇＞銆佺硸鍩哄寲鍜岄洔璩�(zh矛)閫�(j矛n)琛屾湁鏁堝垎鏋愬拰鐩稿皪(du矛)鍚噺娓�(c猫)瀹氥€�

浜�銆�

绲�(ji茅)妲�(g貌u)

鐥涢粸(di菐n)2

浜岀～閸垫槸鍠姉杓曢噸閺堜箣闁撻張鎺ュ崐鑳辨皑閰告畼鍩轰腑鐨勯嵉锛岀偤浜嗙⒑瀹氳泲鐧借唱(zh矛)鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)锛岄鍏堝繀闋堝皣浜岀～閸垫墦闁�(k膩i)锛屼娇鎴愮偤绶氱媭澶氳偨閺堛€備簩纭嵉灏�(du矛)绌�(w臎n)瀹氳泲鐧借唱(zh矛)钘ョ墿鐨勭┖闁撶祼(ji茅)妲�(g貌u)锛屼繚鎸佸叾娲绘€у叿鍌欓噸澶х殑浣滅敤銆備富瑕佹娓�(c猫)鏄偤浜嗭細1锛夊皪(du矛)鏈熸湜浜岀～閸甸€ｆ帴鐨勯(y脿n)璀�锛�2锛夊皪(du矛)浣嶇疆锛堥尟(cu貌)閰嶏級浜岀～閸电殑鐧�(f膩)鐝�(xi脿n)銆傚偝绲�(t菕ng)鐨勪簩纭嵉鍒嗘瀽鑸囦簩纭嵉鐨勭洿鎺ュ垎鏋愭硶灏�(du矛)姣旂櫦(f膩)鐝�(xi脿n)锛屽偝绲�(t菕ng)鐨勬柟寮忛潏鏁忓害宸�銆佸垎鏋愰€氶噺浣�銆佷笉鍚屽垎鏋愪汉鍝￠€犳垚鐨勫垎鏋愮祼(ji茅)鏋滃樊鐣拌純澶с€�

鏂规

鐩存帴閲囬泦闈為倓鍘熻泲鐧借唱(zh矛)钘ョ墿鑲藉湒璩�(zh矛)璀滄暩(sh霉)鎿�(j霉)锛屽苟閫氶亷(gu貌)鍖栬粺浠惰嚜鍕�(d貌ng)鍖栨煡鎵句簩纭嵉銆備簩纭嵉鐨勮В鏋愬皪(du矛)杌熶欢鐨勮姹傚緢楂�锛岄渶瑕佹湁妤靛叾寮�(qi谩ng)澶х殑鏁�(sh霉)鎿�(j霉)铏曠悊鎬ц兘銆侭iopharma Finder杌熶欢婧愪簬钘ヤ紒Amgen 鍏�(n猫i)閮ㄨ嚜鍒剁殑鑲藉湒鍒嗘瀽杌熶欢锛屽洜姝ゅ叾鍔熻兘瑷�(sh猫)瑷�(j矛)寰炰竴闁�(k膩i)濮嬪氨鑱�(t墨ng)寰炰簡涓€绶氬垎鏋愪汉鍝＄殑鍒囧(sh铆)闇€姹傦紝 鑰孴hermo Fisher 鐨勫姞鍏�锛屽墖浣胯粺浠�搴曞堡灏�(du矛)璩�(zh矛)璀滄暩(sh霉)鎿�(j霉)铏曠悊鐨勬€ц兘澶уぇ鎻愰珮銆�

姝ゅ锛岀祼(ji茅)妲�(g貌u)閭勫寘鎷簩绱�(j铆)銆佷笁绱�(j铆)绲�(ji茅)妲�(g貌u)锛屽嵆鍒╃敤铔嬬櫧璩�(zh矛)鐨勫湏浜岃壊鎬у嵆涓嶅皪(du矛)绋卞垎瀛愬皪(du矛)宸﹀彸鍦撳亸鎸厜鍚告敹鐨勪笉鍚屼締(l谩i)閫�(j矛n)琛岀祼(ji茅)妲�(g貌u)鍒嗘瀽锛岀啽绌�(w臎n)瀹氭€у垎鏋愶紙DSC锛夋脯(c猫)瀹歍m鍊肩瓑銆�

涓�銆�

缈昏鍚庝慨椋�

鐥涢粸(di菐n)3

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鏂规

GlycanPac AXH-1/AXR-1 鑹茶瓬鏌辩祼(ji茅)鍚堜簡寮遍櫚闆㈠瓙浜ゆ彌WAX 鍜岃Κ姘寸浉浜掍綔鐢℉ILIC (AXH-1)/ 鍙嶇浉RP (AXR-1) 鍏╃ó淇濈暀姗�(j墨)鐞�锛岃兘澶犵巼鍦板垎鏋愬京(f霉)闆滃绯栭張娣峰悎妯ｆ湰銆傜稉(j墨ng)鍏� HILIC/RP 姗�(j墨)鐞嗗彲浠ユ牴鎿�(j霉)闆昏嵎鏁�(sh霉)銆佹サ鎬с€佸ぇ灏忓(sh铆)鐝�(xi脿n)瀵＄硸閺堝垎闆�锛岃€學AX 姗�(j墨)鐞嗕繚鐣欏拰閬告搰甯惰矤(f霉)闆昏嵎鐨勫绯栭張锛屽皪(du矛)鍚斁娑查吀鐨勫绯栭張鍏锋湁寰堝ソ鐨勫垎闆㈡晥鏋�銆侽rbitrap 鐢变簬鍏锋湁瓒呴珮鍒嗚鲸鐜囧拰瓒呴珮闈堟晱搴︼紝鐒�(w煤)璜栧皪(du矛)浜庡柈鎶楃瓑绱旇泲鐧芥ǎ鍝�銆侀倓鏄叏绱�(x矛)鑳炶泲鐧界瓑寰�(f霉)闆滄ǎ鍝�锛岄兘鏄槸绯栧熀鍖栦綅榛�(di菐n)琛ㄥ緛鐨勫伐鍏�銆�

鍥�銆�

杓旀枡妾㈡脯(c猫)

鐥涢粸(di菐n)4

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鏂规

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